PS100026 | pCMV6-A-GFP Mammalian Expression Vector Clinisciences

Specifications
Product Data
E. coli Selection	Ampicillin (100 ug/mL)
Expression Promoter	CMV
Features	This vector is essentially the same as the pCMV6-Entry vector, except for the alternative selection marker (GFP replacing Neomycin). GFP can be used to monitor transfection efficiency or as a cell sorting marker. TurboGFP (excitation/ emission max = 482/ 502 nm) is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboGFP There is no drug selection marker in this vector for mammalian cells. The vector itself does not contain additional tag. If the insert is shuttled from pCMV6-Entry via SgfI/AscI and RsrII/MluI, it will express non-tagged protein. If the insert is shuttled from the pCMV6-Entry vector using SgfI/AscI and Pme I, then it will express Myc-DDK tagged protein. V2_F Primer can be used to sequence the target sequence after cloning. XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
Shipping	Ambient

Product Manuals
TrueORF and PrecisionShuttle cloning vectors Application Guide
FAQs
FAQ for Tagged Cloning Vectors
SDS for PS100026

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual AAV Biosafety

pCMV6-A-GFP Mammalian Expression Vector