Penicillinase antibody (Peroxidase)
Referência orb344906-25ul
Tamanho : 25ul
Marca : Biorbyt
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Penicillinase antibody (Peroxidase)
Catalog Number: orb344906
| Catalog Number | orb344906 |
|---|---|
| Category | Antibodies |
| Description | Penicillinase antibody (Peroxidase) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | DOT, IP, WB |
| Reactivity | Bacteria |
| Isotype | IgG |
| Immunogen | Penicillinase [Enterobacter cloacae] |
| Concentration | 1.0 mg/mL |
| Dilution range | IP: 1:100, WB: 1:250 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Penicillinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae]. Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined. |
| Conjugation | HRP |
| UniProt ID | P05364 |
| NCBI | AAO24600.1 |
| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | rabbit anti-Penicillinase Antibody Peroxidase Conj |
| Note | For research use only |
| Application notes | Anti-Penicillinase Peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Penicillinase [Enterobacter cloacae] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |