PIEZO1 Mouse mAb
Referência MBS435119-0,05mL
Tamanho : 0.05mL
Marca : MyBiosource
Huvecs transfected with Piezo1-GFP, Staining with mouse anti-human Piezo1 antibody (5 ug/ml, MBS435119). No Permeablized. Recommended dilution: 10 ug/ml
IF-Anti-PIEZO1 Antibody MBS435119
IF-Anti-PIEZO1 Antibody MBS435119
Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling PIEZO1 with MBS435119 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
PIEZO1 Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435119The protein encoded by this gene is a mechanically-activated ion channel that links mechanical forces to biological signals. The encoded protein contains 36 transmembrane domains and functions as a homotetramer. Defects in this gene have been associated with… More details
Product Overview
Online Datasheet in PDFProduct Name
PIEZO1, Monoclonal Antibody
Full Product Name
PIEZO1 Mouse mAb
Product Gene Name
anti-PIEZO1 antibody
Product Synonym Gene Name
FAM38A; KIAA0233
Product Synonym Names
FAM38A, KIAA0233
Clonality
Monoclonal
Isotype
Mouse IgG
Host
Mouse
Species Reactivity
Homo Sapiens
Preparation and Storage
Store at -20 degree C.
Molecular Weight
Predicted MW: 286.6 kDa
Source
This monoclonal antibody is produced by immunizing mice with a polypeptide corresponding to PIEZO1 protein.
Product Advantage
Mass Spectrum approved
Product Categories/Family for anti-PIEZO1 antibody
Cell Marker; Subcellular Marker; Membrane
Applications Tested/Suitable for anti-PIEZO1 antibody
Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FC)
Application Notes for anti-PIEZO1 antibody
Immunoprecipitation: 10 tests
Immunofluorescence: 1:100-1:200
FC: 1:100-1:200
For Western Blot (WB), incubate membrane with diluted antibdoy in 5% w/v nonfat dry milk, 1x TBS, 0.05% Tween-20 at 4 degree C with gentle shaking overnight.
Immunofluorescence: 1:100-1:200
FC: 1:100-1:200
For Western Blot (WB), incubate membrane with diluted antibdoy in 5% w/v nonfat dry milk, 1x TBS, 0.05% Tween-20 at 4 degree C with gentle shaking overnight.
Flow Cytometry (FC/FACS) of anti-PIEZO1 antibody
Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling PIEZO1 with MBS435119 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Other Notes
Small volumes of anti-PIEZO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PIEZO1 purchase
MBS435119 is an antibody targeting PIEZO1. Also known as PIEZO1 Mouse mAb. Reactive in Homo Sapiens. Host: Mouse.
Additional Details
NCBI/Uniprot data below describe general gene information for PIEZO1. It may not necessarily be applicable to this product.
NCBI Official Full Name
piezo-type mechanosensitive ion channel component 1
NCBI Official Synonym Full Names
piezo type mechanosensitive ion channel component 1
NCBI Official Symbol
PIEZO1
NCBI Official Synonym Symbols
DHS; Mib; LMPH3; FAM38A
UniProt Protein Name
Piezo-type mechanosensitive ion channel component 1
UniProt Synonym Protein Names
Membrane protein induced by beta-amyloid treatment; Mib; Protein FAM38A
Protein Family
Piezo-type mechanosensitive ion channel component
UniProt Gene Name
PIEZO1
UniProt Synonym Gene Names
FAM38A; KIAA0233; Mib
NCBI Protein Information
piezo-type mechanosensitive ion channel component 1
UniProt Comments for PIEZO1
Pore-forming subunit of a mechanosensitive non-specific cation channel (PubMed:23479567, PubMed:23695678). Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling (PubMed:20016066). In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation (PubMed:25119035). Plays a key role in blood vessel formation and vascular structure in both development and adult physiology ().
Show more Research Articles on PIEZO1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-PIEZO1 antibody
| Reference Product | PubMed Publications |
|---|---|
| SACS antibody | >3 publications with PIEZO1 and SACS |
| RHAG antibody | >2 publications with PIEZO1 and RHAG |
Diseases associated with anti-PIEZO1 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Pregnancy Complications Antibodies | >16 publications with PIEZO1 and Pregnancy Complications |
| Nervous System Diseases Antibodies | >3 publications with PIEZO1 and Nervous System Diseases |
| Inflammation Antibodies | >2 publications with PIEZO1 and Inflammation |
| Mental Disorders Antibodies | >1 publications with PIEZO1 and Mental Disorders |
| Hypertrophy Antibodies | >1 publications with PIEZO1 and Hypertrophy |
| Breast Neoplasms Antibodies | >1 publications with PIEZO1 and Breast Neoplasms |
| Necrosis Antibodies | >1 publications with PIEZO1 and Necrosis |
Organs/Tissues associated with anti-PIEZO1 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >27 publications with PIEZO1 and Blood |
| Vascular Antibodies | >9 publications with PIEZO1 and Vascular |
| Brain Antibodies | >9 publications with PIEZO1 and Brain |
| Heart Antibodies | >7 publications with PIEZO1 and Heart |
| Muscle Antibodies | >6 publications with PIEZO1 and Muscle |
| Bone Antibodies | >3 publications with PIEZO1 and Bone |
| Bladder Antibodies | >3 publications with PIEZO1 and Bladder |
| Eye Antibodies | >2 publications with PIEZO1 and Eye |
| Skin Antibodies | >2 publications with PIEZO1 and Skin |
| Lung Antibodies | >2 publications with PIEZO1 and Lung |