Pig interleukin 2,IL-2 ELISA Kit

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Citations

Customer Reviews and Q&A

Target Background

Marca : Cusabio

Referência CSB-E06783p-5x96T

Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

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Tamanho : 5x96T

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SDS

CSB-E06783p

Size	96T,5×96T,10×96T

Target Name

interleukin 2

Associated names

IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit

Abbreviation

Uniprot No.

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	104
	Range %	97-111
1:2	Average %	90
	Range %	85-96
1:4	Average %	95
	Range %	88-101
1:8	Average %	89
	Range %	82-96

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	2.687	2.634	2.660	2.531
500	1.707	1.667	1.687	1.558
250	0.996	1.028	1.012	0.883
125	0.664	0.629	0.647	0.518
62.5	0.431	0.421	0.426	0.297
31.2	0.241	0.253	0.247	0.118
15.6	0.198	0.184	0.191	0.062
0	0.133	0.125	0.129

ELISA Data Analysis

Troubleshooting
and FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Description

Interleukin 2 (IL-2) is a cytokine that plays a central role in immune system regulation and T cell activation. Originally discovered as a T cell growth factor, IL-2 is primarily produced by activated CD4+ T helper cells and serves as a key mediator of adaptive immune responses. This cytokine promotes T cell proliferation, differentiation, and survival. It also influences the development and function of regulatory T cells. IL-2 signaling occurs through the IL-2 receptor complex and activates multiple downstream pathways that maintain immune homeostasis and mount effective immune responses...

H Li, R Zhang, J Qu, Y Kang, J Zhang, R Guo,Frontiers in Microbiology,2024
H Han,Journal of applied microbiology,2023
Y Wu,Animal Nutrition,2021
X Li,Animal Nutrition,2020
Wang S,Infect Immun,2020
Zong X, et al,Livestock Science,2018
Lijun Zou.et al,Journal of the Science of Food and Agriculture,2018
Huan-sheng YANG.et al,Zhejiang University-SCIENCE B,2016
Xiong X.et al,J Anim Sci.,2015

There are currently no reviews for this product.

The recovery of pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)

EDTA plasma (n=4)

Function
(From Uniprot)

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Gene References into Functions

We observed that besides TCR stimulation Tregs require IL-2 for activation.
a parameter of early mRNA expression during intestinal ischemia reperfusion injury
The isolation and characterization of interleukin 2 from Tibetan swine are reported.

Subcellular Location

Secreted.

Protein Families

IL-2 family

Database Links

KEGG:

STRING:

UniGene: