Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.
 
> Pitstop 2 [1419320-73-2]

Pitstop 2 [1419320-73-2]

Imprimir Imprimir
Please log in

Referência HY-115604-5mg

Tamanho : 5mg

Marca : MedChemExpress


Description

Pitstop 2 is a clathrin inhibitor which inhibits clathrin-mediated endocytosis (CME) by associating with the terminal domain of clathrin. Pitstop 2 has the potential for anti-cancer research[1][2].

In Vitro

Pitstop 2 (20-40 μM; 30 min) inhibits the endocytosis of transferrin in J774A.1 macrophages and does not compromise cell viability[1].
Pitstop 2 (20-40 μM; 30 min) does not affect the internalization of cholera toxin B in J774A.1 macrophages[1].
Pitstop 2 (0.001-100 μM; 6 h) inhibits the mitotic spindle and impairs mitotic progression in HeLa cells[2].
Pitstop 2 (1-30 μM; 24 h) induces apoptosis and inhibits cell growth in dividing cancer cells[2].
Pitstop 2 (1-30 μM; 48 h) does not affect the growth and viability of non-tumourigenic NIH3T3 fibroblasts[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pitstop 2 Related Antibodies

Cell Viability Assay[2]

Cell Line: HeLa cells
Concentration: 1, 3, 10, 30 μM
Incubation Time: 24 hours
Result: Reduced the total number of viable HeLa cells markedly in a dose-dependent manner.
Masse moléculaire

473.36

Formule

C20H13BrN2O3S2
CAS No.
Appearance

Solid

Color

Yellow to brown

SMILES

O=S(C1=C2C=CC=CC2=CC=C1)(NC(S/C3=C\C4=CC=C(Br)C=C4)=NC3=O)=O
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

*In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Solvant et solubilité
In Vitro: 

DMSO : 50 mg/mL (105.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1126 mL 10.5628 mL 21.1256 mL
5 mM 0.4225 mL 2.1126 mL 4.2251 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.28 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Pureté et documentation
Références

Você também pode estar interessado nos seguintes produtos:



Referência
Descrição
Cond.
Price Bef. VAT
HY-13404-5mg
Capmatinib [1029712-80-8]
 5mg 
HY-15304-10mg
Dynasore [304448-55-3]
 10mg 
HY-17364-10mg
Temozolomide [85622-93-1]
 10mg