Polyclonal Antibody to Neurofilament NF-M

Figure-1: Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M,(34-1087), dilution 1:1,000 in red, and costained with mouse mAb to CNP, dilution 1:500 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. The NF-M antibody labels the network of axons of basket neurons and other neurons. The CNP antibody stains oligodendrocytes, cells that create myelin sheaths around axons.