Primary Human Glomerular Mesangial Cells (ACBRI 127) were initiated by elutriation of dispase dissociated decapsulated glomeruli isolated from normal human cortical kidney tissue.

Cell Initiation

These cells were originated using Neo Biotech Complete Serum-Free Medium , and subsequently grown and passaged in Neo Biotech Complete Medium . They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Neo Biotech Cell Freezing Medium™ . This vial will initiate a Passage 4 cell culture in a 75cm² flask. Each vial contains more than 1 x 10⁶cells.

In addition to cryopreserved vials, these cells also are available in 25cm² and 75cm² proliferating cell culture flasks (US domestic market only).

Companion Products

Each vial of cells is shipped with vials of Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.

These cells are qualified for use with:

Complete Classic Medium Kit with Serum and CultureBoost™

ADD TO CART

The System™

ADD TO CART

Passage Reagent Group™

ADD TO CART

Standard Tests

TEST	RESULTS
HIV Serologic Test (donor level HIV AB EIA)	Negative
HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Hepatitis B (HBV) PCR Test (frozen cells)	Negative
Hepatitis C (HCV) PCR Test (frozen cells)	Negative
Mycoplasma spp. (frozen cells) (ATCC method by CLIA Licensed Clinical Lab)	Negative

Miscellaneous Tests

TEST	RESULTS
Inducible expression of CD 62E	< 2% positive by immunofluorescence
Cytoplasmic Vimentin Intermediate Filaments	> 98% positive by immunofluorescence
Cytoplasmic Alpha-Actinin Microfilaments	> 98% positive by immunofluorescence
Cytoplasmic Fibronectin	> 98% positive by immunofluorescence

Citations
"Study of the target effect of mannose modified liposomes on diabetic rat kidney based on GLUT" Yang et al. Journal of Drug Delivery Science and Technology, 2020
"Cell Surface Protein Disulfide Isomerase Regulates Natriuretic Peptide Generation of Cyclic Guanosine Monophosphate" Pan et al. PLoS One, 2014.

"The combined role of galactose-deficient IgA1and streptococcal IgA-binding M protein in inducing IL-6 and C3 secretion from human mesangial cells: implications for IgA nephropathy" Schmitt et al. J Immunology, 2014.
"Promoter methylation confers kidney-specific expression of the Klotho gene" Azuma et al. FASEB J, 2012.
"Nicotine worsens the severity of nephropathy in diabetic mice: implications for the progression of kidney disease in smokers" Hua et al. Renal Physiology, 2010.
"Nicotine: the link between cigarette smoking and the progression of renal injury?". Jaimes et al. Am J Physiol Heart Circ Physiol, 2007.
"Blocking of α5 integrin stimulates production of TGF-β and PAI-1 by human mesangial cells" Matsumoto et al. Elsevier, 2003

Neo Biotech cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Neo Biotech cells may not be genetically altered in any way without prior written permission from Neo Biotech. Use of Neo Biotech materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.

Neo Biotech was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Neo Biotech provides cells and cell culture products to other research entities - public and private.

Please read our Terms and Conditions

Primary Human Glomerular Mesangial Cells (Set with NeoBAC and NeoBoost)

Referência NB-11-0001

Marca : Neo Biotech

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