Anti-SLU7 Antibody

Western blot analysis of SLU7 expression in SGC7901 (A), LO2 (B), mouse kidney (C) whole cell lysates. (Predicted band size: 68 kD; Observed band size: 80 kD)

Immunofluorescent analysis of SLU7 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).