Rat Kappa Antibody : AP
Referência OASB00800
Tamanho : 1.0mL
Marca : Aviva Systems Biology
Rat Kappa Antibody : AP (OASB00800)
| Datasheets/Manuals | Printable datasheet for Rat Kappa Antibody : AP (OASB00800) |
|---|
| Tested Species Reactivity | Rat |
|---|---|
| Predicted Species Reactivity | Rat |
| Clonality | Monoclonal |
| Clone | K4F5 |
| Isotype | IgG2a |
| Host | Mouse |
| Conjugation | AP |
| Application | IHC-F, WB |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Rat kappa hybridoma |
| Concentration | Lot specific |
| Specificity | Kappa |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested as a second step reagent by flowcytometry and/or ELISA to conform to characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: ELISA: Purified antibody Fluorescein Conjugate R-phycoerythrin conjugate Alkaline phosphatase conjugate Horseradish peroxidase conjugate <= 3 ug/106 cells <= 3 ug/106 cells <= 0.2 ug/106 cells 1:1,000-1:2,000 1:2,000-1:8,000 |
| Application Info | Enzyme-Linked-Immunosorbent-Assay (ELISA), Identification and enumeration of rat kappa light chain+ cells by flow cytometry |
| Other Applications Data | Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The BIOT conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3; store at 2- 8 C - The R- PE conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent; store at 2- 8 C Do Not Freeze - Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label ifstored as directed. |
| Gene Symbol | IGKC |
|---|---|
| Gene Full Name | Ig kappa chain C region |
| Alias Symbols | Igk@, D4Mit8 |
| NCBI Gene Id | 500183 |
