Recombinant Histone H3 (H3)
Referência MBS2153809-5mg
Tamanho : 5mg
Marca : MyBiosource
Figure. SDS-PAGE
Histone H3 (H3) Recombinant Protein
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2153809MBS2153809 is a recombinant protein targeting H3. Also known as Recombinant Histone H3 (H3). Host: E coli. More details
Product Overview
Online Datasheet in PDFProduct Name
Histone H3 (H3), Recombinant Protein
Full Product Name
Recombinant Histone H3 (H3)
Product Gene Name
H3 recombinant protein
Product Synonym Names
Histone H3; H3 recombinant protein (prokaryotic), Human
Host
E coli
Form/Format
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Freeze-dried powder
Freeze-dried powder
Purity/Purification
Greater than 90% by SDS-PAGE
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Stable for 12 months if correctly stored.
Stable for 12 months if correctly stored.
Molecular Weight
15,404 Da
Sequence Positions
Met1~Ala136
Chromosome Location
Chromosome: 6; NC_000006.11 (26020718..26021186). Location: 6p22.1
OMIM
602810
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Expression System
Prokaryotic expression
Species
Human
Tag
N-terminal His
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
11.7
Subcellular Location
Nucleus, Chromosome
Research Area
Developmental science
Product Categories/Family for H3 recombinant protein
Prokaryotic proteins
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for H3 recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Other Notes
Small volumes of H3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1, H3a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
UniProt Protein Name
Histone H3.1
UniProt Synonym Protein Names
Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
Protein Family
Tyrosine phosphatase
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
UniProt Entry Name
H31_HUMAN
NCBI Protein Information
histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A
NCBI Summary for H3
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for H3
H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 6p22.2
Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus
Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity
Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly; DNA methylation on cytosine
Show more Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 6p22.2
Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus
Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity
Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly; DNA methylation on cytosine
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with H3 recombinant protein
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|---|---|
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| Alcoholism Pathway antibodies | Alcoholism Pathway Diagram |
| Amyloids Pathway antibodies | Amyloids Pathway Diagram |
| Disease Pathway antibodies | Disease Pathway Diagram |
| Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies | Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram |
| Gene Expression Pathway antibodies | Gene Expression Pathway Diagram |
| Hemostasis Pathway antibodies | Hemostasis Pathway Diagram |
| Meiosis Pathway antibodies | Meiosis Pathway Diagram |
| Meiotic Recombination Pathway antibodies | Meiotic Recombination Pathway Diagram |
| RNA Polymerase I Chain Elongation Pathway antibodies | RNA Polymerase I Chain Elongation Pathway Diagram |