Recombinant Renilla reniformis Luciferase
Recombinant Renilla Luciferase Protein
Recombinant Renilla Luciferase Protein
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Species | Renilla Reniformis (sea Pansy) |
| Protein Name / Synonyms | Rluc |
| Expressed Region | Full- length |
| Expression System | E. coli |
| Clonality | Polyclonal |
| Tag | C-terminal 6x histidine tag |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | > 95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 30 |
| Format | Lyophilized powder |
| Formulation | Lyophilized. Renilla luciferase protein was constituted in a 0.2 µm filtered phosphate-buffered saline (1x PBS) before lyophilized. |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1 ~ 1 mg/mL with sterile ice-cold deionized water or 1x Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers. |
| Endotoxin Level | Not determined |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Activity
Using the Renilla Lucifearse assay kit (Promega), the measured net Relative Light Unit (RLU) was approximately 5 x 1010 / ug of enzyme.
Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
documents
citations
Bioprofiling of the EU-OPENSCREEN European Chemical Biology Library – an open science initiative in chemical biology and early drug discovery
- Sample Type:
- Other / Unspecified (compound library screening renilla luciferase assay C2)
- Year:
- 2026
- Journal Name:
- Research Square
Interference of metal ions on the bioluminescent signal of firefly, Renilla, and NanoLuc luciferases in high-throughput screening assays
- Year:
- 2024
- Journal Name:
- Frontiers in Chemistry
- PubMed ID:
- 39659874
Transcriptional Inhibition of MicroRNA miR-122 by Small Molecules Reduces Hepatitis C Virus Replication in Liver Cells
- Year:
- 2022
- Journal Name:
- Journal of Medicinal Chemistry
Production of human translation-competent lysates using dual centrifugation
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (translation-competent lysates immunoblot standard curve)
- Year:
- 2022
- Journal Name:
- RNA biology
- PubMed ID:
- 34965175
Copper-mediated oxidation of imidazopyrazinones inhibits marine luciferase activity
- Year:
- 2022
- Journal Name:
- Luminescence
A High-Throughput Drug Screening Strategy for Detecting Rhodopsin P23H Mutant Rescue and Degradation
- Sample Type:
- N/A (Recombinant Renilla Luciferase)
- Year:
- 2015
- Journal Name:
- Investigative Opthamology and Visual Science
- PubMed ID:
- 25783607
New bioluminescent coelenterazine derivatives with various C-6 substitutions.
- Species:
- Human
- Year:
- 2017
- Journal Name:
- Organic and Biomolecular Chemistry
Measuring inhibition of HIV replication by ex vivo CD8+ T cells
- Year:
- 2014
- Journal Name:
- Journal of Immunological Methods
