RH FGF-7 / KGF (AF)
Referência 100-187AF-500UG
Tamanho : 500UG
Marca : FUJIFILM
Recombinant Human FGF-7 / KGF
Fibroblast growth factor 7 (FGF-7), also known as keratinocyte growth factor (KGF), is a potent mitogen that regulates epithelial cell migration and differentiation. FGF-7 is produced by mesenchymal cells and binds in high affinity to a splice variant of FGF receptor 2 (FGFR2-IIIb). The mitogenic activity of FGF-7 acts predominantly on keratinocytes, but not on fibroblast or endothelial cells. FGF-7 expression is upregulated after acute and chronic injury, suggesting that FGF-7 functions during the healing of injured epithelial cells. FGF-7 also induces the formation of the apical ectodermal ridge during limb development.
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SDS-PAGE Showing a Single Prominent Band at ~ 15.7/31.3kDa
Figure 1. rh IL-17A (1μg) was resolved with SDS-PAGE reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at ~ 16/32kDa
Cell-Based Proliferation Assay
Figure 2. Recombinant Human FGF-7 / KGF induced proliferation in 4MBr-5 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | KGF |
| Accession Number | P21781 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 19 kDa (164 aa) |
| AA Sequence | MACNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium phosphate, 0.5 M sodium chloride, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL (reconstitution at concentrations > 0.1 mg/mL may result in a precipitate) |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 90% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | 4MBr-5 cell proliferation | ≤ 60 ng/mL; ≥ 1.7 x 104 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.