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RNAse Inhibitor

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Referência P0270

Tamanho : 10000U

Marca : Canvax Biotech


Canvax RNAse Inhibitor 10,000 U (20 U/µL) for molecular biology research

RNAse Inhibitor

High-purity recombinant RNase Inhibitor for efficient protection of RNA from degradation in RT, PCR, qPCR, and IVT applications.

RNase Inhibitor is a recombinant protein that protects RNA from degradation by RNases A, B, and C. It binds the enzymes with high affinity, preserving RNA integrity during reactions.

This reagent ensures stable and contamination-free RNA handling, making it essential for reverse transcription, RNA amplification, and in vitro transcription workflows.

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(2 customer reviews)
10,000 U

SKU: P0270 Categories: In Vitro Transcription, Enzymes

Detailed information:

Advantages & Features

  • Recombinant protein expressed in E. coli, free of animal components
  • Strong inhibition of RNases A, B, and C
  • Compatible with reverse transcription, qPCR, PCR, and IVT
  • Reliable: stringent quality control standards to guarantee lot-lot consistency.
  • High Quality: free of DNA exo- and endonuclease, and RNase contamination.

Specifications

Unit Definition
1 U of RNAse inhibitor is defined as an amount of enzyme that blocks activity of 5 ng of RNase A in 50%.

Stability

The enzyme is stable up to 58°C and DTT concentration ranges ( 0.5–1 mM DTT presence is essential for optimal activity). Inactivation of the enzyme occurs at 65°C. The enzyme is active over a broad pH range (pH 5.5 – 9.0).

Concentration: 40 U/µL

Purity: >95% by SDS-PAGE

Includes

Includes for 10,000 U:
– 10,000 U RNAse Inhibitor (40 U/μL)

Download documentation

Applications

– Inhibition of RNA degradation in a variety of Molecular Biology applications:

  • cDNA library construction
  • RT-PCR.
  • RNA-seq sample preparation
  • In vitro transcription/translation systems.
  • Isolation and storage of RNA.
  • RNA labeling and probe synthesis

Tables & Figures

Quality Control

  • Free of detectable DNA exo- and endonuclease, and RNase activities.
  • Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.

Advice

For optimal activity, include RNase Inhibitor in reaction mixtures prior to adding RNA samples. Avoid repeated freeze-thaw cycles.

Storage & Shipping

  • Shipped in: Gel pack.
  • Storage: -20 °C.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

Synonym(s)

RNase inhibitor protein, Ribonuclease inhibitor, RNase blocker, RNA protection enzyme, Recombinant RNase inhibitor

Also known as:

  • Spanish: Inhibidor de RNasa, Inhibidor de ribonucleasas, Protector de ARN
  • French: Inhibiteur de RNase, Inhibiteur de ribonucléases, Protecteur d’ARN
  • German: RNase-Inhibitor, Ribonuklease-Inhibitor, RNA-Schutzprotein
  • Italian: Inibitore di RNasi, Inibitore delle ribonucleasi, Protezione RNA

FAQs

Q1: What is the difference between recombinant and human RNase Inhibitor?
A1: Recombinant RNase Inhibitor shows higher thermal stability and eliminates the risk of animal-derived contaminants.

Q2: Can it be used in one-step RT-qPCR kits?
A2: Yes, it is fully compatible with both one-step and two-step RT-qPCR.

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