SMCC [64987-85-5]
Referência HY-42360-100mg
Tamanho : 100mg
Marca : MedChemExpress
SMCC is a protein crosslinker. SMCC-conjugated antigen coupled spleen cells to induce antigen-specific immune responses.
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SMCC Chemical Structure
CAS No. : 64987-85-5
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Description |
SMCC is a protein crosslinker. SMCC-conjugated antigen coupled spleen cells to induce antigen-specific immune responses[1]. |
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In Vitro |
SMCC or sulfo-SMCC can use to conjugate keyhole limpet hemocyanin (KLH) and ovalbumin (OVA). In the the conjugation system, 300 μl KLH or OVA (3 mg) and 30 μl SMCC or Sulfo-SMCC (the final concentration of 0.1 mM) are added in PBS with final volume of 3 ml[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
Using the SMCC-conjugation method is able to induce potent immune responses to whole protein or peptide antigens by injection of antigen-coupled syngeneic splenic mononuclear cells in mice[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Essai clinique |
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Masse moléculaire |
334.32 |
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Formule |
C16H18N2O6 |
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CAS No. |
64987-85-5 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
O=C(C1CCC(CN2C(C=CC2=O)=O)CC1)ON3C(CCC3=O)=O |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Solvant et solubilité |
In Vitro:
DMSO : 25 mg/mL (74.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : < 0.1 mg/mL (insoluble) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation |
Purity: 99.25% |
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Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.9911 mL | 14.9557 mL | 29.9115 mL | 74.7787 mL |
5 mM | 0.5982 mL | 2.9911 mL | 5.9823 mL | 14.9557 mL | |
10 mM | 0.2991 mL | 1.4956 mL | 2.9911 mL | 7.4779 mL | |
15 mM | 0.1994 mL | 0.9970 mL | 1.9941 mL | 4.9852 mL | |
20 mM | 0.1496 mL | 0.7478 mL | 1.4956 mL | 3.7389 mL | |
25 mM | 0.1196 mL | 0.5982 mL | 1.1965 mL | 2.9911 mL | |
30 mM | 0.0997 mL | 0.4985 mL | 0.9970 mL | 2.4926 mL | |
40 mM | 0.0748 mL | 0.3739 mL | 0.7478 mL | 1.8695 mL | |
50 mM | 0.0598 mL | 0.2991 mL | 0.5982 mL | 1.4956 mL | |
60 mM | 0.0499 mL | 0.2493 mL | 0.4985 mL | 1.2463 mL |
SMCC Related Classifications
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
SMCC64987-85-5ADC LinkerAntibody-drug conjugates linkerInhibitorinhibitorinhibit