Succinate Dehydrogenase Activity Colorimetric Assay Kit

Succinate Dehydrogenase (SDH) or respiratory complex II or succinate-coenzyme Q reductase (SQR) is an enzyme complex that is present in the inner mitochondrial membrane. SDH is involved in both the electron transport chain and citric acid cycle. In mammals and many bacteria, SDH consists of 2 hydrophobic membrane anchor subunits (SDHC and SDHD) and 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB (iron-sulfur protein). SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone. In humans, SDH deficiency can lead to a variety of phenotypes including myopathy, tumor formation and Leigh syndrome, a neurometabolic disorder. SDH can also prevent the generation of ROS (reactive oxygen species). Measurement of SDH activity has wide applications. The Succinate Dehydrogenase Activity Colorimetric Assay Kit provides a sensitive, fast and simple way for detection of SDH activity in various samples based on colorimetric method. In the assay, SDH converts succinate to fumarate and transfers the electron to an artificial electron acceptor (SDH Probe), which transforms the color from blue to a colorless product (depending upon the sample enzymatic activity). The assay is high-throughput adaptable. The kit can detect less than 0.1 mU SDH activity in a variety of samples.