TNF RII Protein, Human, Recombinant (hFc)

1. Loaded Recombinant Human TNF RII (C-Fc) on Pro-A Biosensor, can bind Recombinant Human TNF alpha with an affinity constant of 25.2 pM as determined in BLI assay. (Regularly tested) 2. Measured by its ability to induce NF-κB reporter activity in HEK293 human embryonic kidney cells. Recombinant Human TNF RII inhibits a constant dose of 0.5ng/mL of Recombinant TNF alpha. The IC50 for this effect is 2.5 ng/mL. (Regularly tested) 3. Immobilized Recombinant Human TNF alpha at 5μg/ml (100 μl/well) can bind Recombinant Human TNF RII (C-Fc) . The ED50 of Recombinant Human TNF RII (C-Fc)  is 15.35 ng/ml. (Regularly tested)

TNF RII Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 60-90 KDa and the accession number is P20333.

Human

HEK293 Cells

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) is a member of the tumor necrosis factor receptor superfamily. Human TNF RII contains four cysteinerich repeats in its ECD, which shares 58% and 56% amino acid sequence identity with the mouse and rat orthologs, respectively.TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes,neurons, oligodendrocytes, cardiac myocytes, thymocytes, and mesenchymal stem cells.TNF RII binds to the membranebound forms of TNFα and Lymphotoxinα/TNFβ;soluble TNF is thought to signal predominately through TNF RI.Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors.