Tricine is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good’s buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm, chemically stable, and stable to temperature and concentration changes.

Synonyms:
N-Tris(Hydroxymethyl)methylglycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine

CAS No:
5704-04-1

Molecular Formula:
C6H13NO5

Molecular Weight:
179.2

Purity:
≥99%

Appearance:
White crystalline powder

Solubility (0.1M in H2O):
Colorless, clear, complete

pH:
4.0-6.0

pKa:
8.0-8.3 (25°C)

A260:
≤0.04

A280:
≤0.02

Loss on Drying:
≤1%

RNase:
None Detected

DNase:
None Detected

Protease:
None Detected

Endotoxin:
≤ 0.1EU/mg

Storage:
RT