WI-38 cells
Referência T0016037
Tamanho : OneFrozenvial
Disease:
Normal
Origin:
The WI-38 human diploid cell line was derived by Leonard Hayflick from normal embryonic (3 months gestation) lung tissue.
Species:
Homo sapiens, human Lung Fibroblast Adherent Normal diploid Female, Caucasian, 3 months gestation fetus AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C. Complete growth culture medium supplemented with 7.5% (v/v) DMSO I Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 11,12 D5S818: 10 D7S820: 9,11 THO1:8,9.3 TPOX: 8 vWA: 19,20 If use of this culture results in a scientific publication, it should be cited in the publication as: WI-38 (AddexBio T0016037) Tissue:
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Citation Format:
References for WI-38 cells:
1. HAYFLICK L, MOORHEAD PS. The serial cultivation of human diploid cell strains. Exp Cell Res. 1961 Dec;25:585-621. PMID: 13905658