F(ab) fragmented secondary antibodies
Fab and F(ab')2 fragments are two types of fragmented secondary antibodies that are commonly used in various applications. Here are some key points about F(ab) fragments:
- F(ab) fragments are monovalent antibody fragments that contain only one antigen-binding Fab portion and lack the Fc region. They are generated by papain digestion of whole IgG antibodies to remove the Fc region and the entire hinge region.
- F(ab) fragments have a molecular weight of about 50 kDa, which is smaller than whole IgG antibodies and F(ab')2 fragments. This allows for better penetration into tissues and reduces nonspecific binding to Fc receptors on live cells or to Protein A/G.
- F(ab) fragments are useful for blocking endogenous immunoglobulins on cells, tissues, or other surfaces, and for blocking the exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species. They can also be used to Fab-label primary antibodies prior to incubation with the experimental sample.
- F(ab) fragments are monovalent and have lower avidity than F(ab')2 fragments, which may affect their sensitivity and specificity in certain applications.
Resultado da sua pesquisa : 865 produto encontrado
Refine sua procura :
RUOCE / IVD
- Unconjugated 316
- FITC 54
- HRP 49
- Biotin 41
- TRITC 22
- Alexa Fluor 350 18
- Alexa Fluor 405 18
- Alexa Fluor 532 18
- Alexa Fluor 555 18
- Alexa Fluor 568 18
- Alexa Fluor 633 18
- Alexa Fluor 647 18
- Alexa Fluor 660 18
- Alexa Fluor 680 18
- Alexa Fluor 750 18
- AP 16
- AMCA 14
- Alexa Fluor 790 14
- Cy3 14
- Cy5 14
- Cy5.5 14
- Cy7 14
- Red 14
- Alexa Fluor 488 12
- Alexa Fluor 594 12
- PE 7
- APC 6
- AbFluor™ 488 6
- AbFluor™ 594 6
- Allophycocyanin 1
- PerCp 1
- R-Phycoerythrin 1
- human 596
- rabbit 34
- rat 28
- mouse 27
- goat 23
- sheep 21
- chicken 20
- hamster 20
- guinea pig 19
- pig 2
- cat 1
- cow 1
- dog 1
- horse 1
- Secondary antibody
- Secondary Antibody 4
- goat 373
- mouse 281
- rabbit 89
- donkey 30
- human 28
- monkey 6
- rat 6
- guinea pig 3
- chicken 1
- IHC 684
- IF 584
- FC 560
- ELISA 470
- WB 215
- FLISA 73
- Microscopy 53
- ICC 38
- IP 26
- Dot blot 16
- Polyclonal 415
- Monoclonal 210
- polyclonal 76
- NI 6014 (HP 6014) 6
- MA2 4
- 4A11 2
- NI 330 (HP 6050) 2
- RMG01 2
- RMG05 2
- 1105CT1.1.1 1
- 7HA13 1
Referência
Descrição
Cond.
Price Bef. VAT
‹
›