5-Nucleotidase, 5-NT BioAssay™ ELISA Kit (Mouse)

For the quantitative determination of mouse Tumor necrosis factor alpha concentrations in serum, plasma, and tissue homogenates.

Sensitivity:
0.031mU/ml

Detection Range:
0.125-8mU/ml

Specificity:
This assay has high sensitivity and excellent specificity for detection of mouse 5-NT. No significant cross-reactivity or interference between mouse 5-NT and analogues was observed.

Assay Principle:
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 5-NT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 5-NT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5-NT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5-NT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Kit Components:
Microtiter Strips, 1x96 wells
Standard (Freeze dried), 2xvial
Biotin-antibody (100X), 1x120ul
HRP-avidin (100X), 1x120ul
Biotin-antibody Diluent, 1x15ml
HRP-avidin Diluent, 1x15ml
Sample Diluent, 1x50ml
Wash Buffer (25X), 1x20ml
TMB Substrate, 1x10ml
Stop Solution: 1x10ml

Storage and Stability:
Store all kit components at 4°C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.