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> 6-Benzylaminopurine [1214-39-7]

6-Benzylaminopurine [1214-39-7]

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Referência HY-B0941-1mL

Tamanho : 10mM/1mL

Marca : MedChemExpress


Description

6-Benzylaminopurine (Benzyladenine) is a cytokinin that causes plant growth and development by stimulating cell division and inhibiting respiratory kinases, thereby prolonging the preservation of green vegetables[1].

IC50 & Target

Cytokinin

 

Cellular Effect
Cell Line Type Value Description References
HL-60 IC50
> 100 μM
Compound: BAP, benzylaminopurine
Antiproliferative activity against human HL60 cells
Antiproliferative activity against human HL60 cells
[PMID: 17300934]
Oocyte IC50
200 μM
Compound: 9
Inhibitory activity against purified cdc2 p34/Cyclin B obtained from M phase oocytes of the starfish Marthasterias glacialis.
Inhibitory activity against purified cdc2 p34/Cyclin B obtained from M phase oocytes of the starfish Marthasterias glacialis.
[PMID: 9046330]
RD CC50
547 μM
Compound: 35
Cytotoxicity against human RD cells
Cytotoxicity against human RD cells
[PMID: 26854380]
RD EC50
0.42 μM
Compound: 35
Antiviral activity against Enterovirus 71 infected in human RD cells assessed as cell viability after 3 days by MTS assay
Antiviral activity against Enterovirus 71 infected in human RD cells assessed as cell viability after 3 days by MTS assay
[PMID: 26854380]
In Vivo

6-Benzylaminopurine (100 μM, when the cotyledons were fully expanded and the frst true leaf was just emerged, each seedling was sprayed every other day until it reached 5-true-leaf stage. Then, each plant was sprayed twice per week until fruit set) results in an increase in wart density by 107.8% in Cshec2 knockout fruits of cucumber[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Masse moléculaire

225.25

Formule

C12H11N5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C12=NC=NC(NCC3=CC=CC=C3)=C1NC=N2
Initial Source

Apis cerana
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvant et solubilité
In Vitro: 

DMSO : 50 mg/mL (221.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4395 mL 22.1976 mL 44.3951 mL
5 mM 0.8879 mL 4.4395 mL 8.8790 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Pureté et documentation
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