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> Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Over-expression Lysate

Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Over-expression Lysate

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Referência LY402777

Tamanho : 100ug


Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Over-expression Lysate

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SKU
LY402777
Transient overexpression lysate of patatin-like phospholipase domain containing 2 (PNPLA2) (as WB positive control)
 

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Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205708
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_020376, NP_065109
Synonyms 1110001C14Rik; ATGL; FP17548; iPLA2zeta; PEDF-R; TTS-2.2; TTS2
Predicted MW 55.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_065109
Locus ID 57104
Cytogenetics 11p15.5
Protein Families WB
Product Manuals
  • Western Blot Protocols
FAQs
  • Over-expression Cell Lysate
SDS
SKU Description Size
PH305708 PNPLA2 MS Standard C13 and N15-labeled recombinant protein (NP_065109) 10 ug
LC402777 Lyophilized PNPLA2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
TP305708 Recombinant protein of human patatin-like phospholipase domain containing 2 (PNPLA2), 20 µg 20 ug

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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