Orb348646-10x500ml | Blockout Universal Blocking Buffer For Western

Blockout Universal Blocking Buffer For Western Blotting

SKU: orb348646

Description

Blockout Universal Blocking Buffer For Western Blotting

Research Area

Metabolism Research

Images & Validation

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Application Notes	BlockOut allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with IRDye 800 and IRDye 700DX (Licor), Cy2, Cy3, Cy3.5, Cy5 and Cy5.5 (GE Healthcare), DyLight405, DyLight 549, DyLight 649, DyLight 680, and DyLight 800 (Thermo Fisher/Pierce) and Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 647 and Alexa Fluor 680 (Invitrogen/Molecular Probes) and ATTO (Atto-Tec) have been validated on various platforms using this product with superior results compared to other commercially available products. In the infrared range, where little to no autofluorescence occurs, specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. This allows for detection levels in the picogram range which rivals the sensitivity of chemiluminescence on film for western blotting. Superior results are also seen when this product is used for simultaneous labeling (multiplex) in western blots or microscopy using various fluorochrome combinations for multicolor imaging. Membranes blocked with BlockOut can be dried and are very stable.

Application Notes

BlockOut allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with IRDye 800 and IRDye 700DX (Licor), Cy2, Cy3, Cy3.5, Cy5 and Cy5.5 (GE Healthcare), DyLight405, DyLight 549, DyLight 649, DyLight 680, and DyLight 800 (Thermo Fisher/Pierce) and Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 647 and Alexa Fluor 680 (Invitrogen/Molecular Probes) and ATTO (Atto-Tec) have been validated on various platforms using this product with superior results compared to other commercially available products. In the infrared range, where little to no autofluorescence occurs, specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. This allows for detection levels in the picogram range which rivals the sensitivity of chemiluminescence on film for western blotting. Superior results are also seen when this product is used for simultaneous labeling (multiplex) in western blots or microscopy using various fluorochrome combinations for multicolor imaging. Membranes blocked with BlockOut can be dried and are very stable.

Key Properties

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Purity	BlockOut Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store Blocking Buffer at 4° C prior to opening. DO NOT FREEZE.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: Thimerosal is added as an antimicrobial agent.; Buffer: See application note.
Concentration	1X
Expiration Date	6 months from date of receipt.
Hazard Information	Non-Toxic
Disclaimer	For research use only

Alternative Names

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Multiplex Blocking Buffer, Immunoblot Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, Western Blot Blocking Buffer, Alexa Dye Blocking Buffer, DyLight Blocking Buffer, colorimetric, chemiluminescent blocking buffer, WB block, BlockOut

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