c-Kit antibody [ACK2] (APC-Cy7)
Referência GTX01469-15-100ug
Tamanho : 100ug
Marca : Genetex
Host | Rat |
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Clonality | Monoclonal |
Clone Name | ACK2 |
Isotype | IgG2b |
Application | FACS |
Reactivity | Mouse |
Package | 100 μg |
View product citations for antibody GTX01469-15 on CiteAb
PRODUCT
Summary
The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application | Recommended Dilution |
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FACS | Assay dependent |
Not tested in other applications.
Calculated MW
109 kDa. ( Note )
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Allophycocyanin-Cyanine7 (APC-Cy7)
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Cellular Localization
Cell membrane,Cytoplasm
Background
The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
DATA IMAGES
GTX01469-15 FACS Image FACS analysis of mouse C57Bl/6 bone marrow cells using GTX01469-15 c-Kit antibody [ACK2] (APC-Cy7). |
Clone Reference
2012; Serotonin 5-HT2B receptors are required for bone-marrow contribution to pulmonary arterial hypertension.
2012; Leukocyte-associated Ig-like receptor-1-deficient mice have an altered immune cell phenotype.
2011; Targeting the CXCR4-CXCL12 axis mobilizes autologous hematopoietic stem cells and prolongs islet allograft survival via programmed death ligand 1.
2011; Neutrophil survival and c-kit(+)-progenitor proliferation in Staphylococcus aureus-infected skin wounds promote resolution.
2011; Inhibition of ovarian KIT phosphorylation by the ovotoxicant 4-vinylcyclohexene diepoxide in rats.
Submit a Reference REVIEW
Clone Reference
2012; Serotonin 5-HT2B receptors are required for bone-marrow contribution to pulmonary arterial hypertension.
2012; Leukocyte-associated Ig-like receptor-1-deficient mice have an altered immune cell phenotype.
2011; Ano1 as a regulator of proliferation.
2011; Targeting the CXCR4-CXCL12 axis mobilizes autologous hematopoietic stem cells and prolongs islet allograft survival via programmed death ligand 1.
2011; Neutrophil survival and c-kit(+)-progenitor proliferation in Staphylococcus aureus-infected skin wounds promote resolution.
2011; Inhibition of ovarian KIT phosphorylation by the ovotoxicant 4-vinylcyclohexene diepoxide in rats.
2006; Kit ligand and c-Kit are expressed during early human ovarian follicular development and their interaction is required for the survival of follicles in long-term culture.
SDS | |
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Sodium Azide.pdf |