Carnosine ELISA Kit
Referência OKCD02258
Tamanho : 96Wells
Marca : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for Carnosine ELISA Kit (OKCD02258) |
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Predicted Species Reactivity | All Species | ||||||||||||||||||||||||
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Application | ELISA-Comp | ||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||||
ELISA Kit Duration | 2h | ||||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Carnosine (Car) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Carnosine (Car) and unlabeled Carnosine (Car) (Standards or samples) with the pre-coated antibody specific to Carnosine (Car). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Carnosine (Car) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Carnosine (Car) in the sample. | ||||||||||||||||||||||||
ELISA Kit Range | 11.11-900ng/mL | ||||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carnosine (Car) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carnosine (Car) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | CAS: 305-84-0 PubChem: 439224 | ||||||||||||||||||||||||
Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||||
Sensitivity | 3.53 ng/mL | ||||||||||||||||||||||||
Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
Gene Symbol | CAR |
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Alias Symbols | L-Carnosine; Carnosine; Beta-Alanyl-L-histidine; 305-84-0; Ignotine |
Protein Name | Carnosine |
Description of Target | L-Carnosine is a naturally occurring dipeptide neuropeptide found in muscles. |