Feline Coronavirus Mouse Monoclonal Antibody [Clone ID: FIPV3-70]

Specifications

Product Data
Clone Name	FIPV3-70
Applications	ELISA, FC, IF, IHC, WB
Recommended Dilution	Flow Cytometry. Immunofluorescence. ELISA: This antibody can be used as a Capture reagent in ELISA assays in combination with AM01094BT-N as a detection reagent. Western Blot: Western blotting, using this antibody under reducing conditions, results in a specific band of 50-56 kDa that represents the nucleocapsid. Immunohistochemistry on Frozen and Paraffin Sections: Microwave induced antigen retrieval in 10 mM citrate buffer is recommended prior to staining of paraffin embedded sections.
Reactivities	Feline Coronavirus, Feline Infectious Peritonitis Virus, Canine Coronavirus, Transmissable Gastroenteritis Virus, Bovine Coronavirus
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Coronavirus cocktail.
Specificity	This antibody reacts with Feline infectious peritonitis virus (FIPV) type 1 and 2 and is known to be specific for the nucleocapsid. It is also known to react with canine coronavirus (CCV), pig coronavirus transmissable gastroenteritis virus (TGEV) and ferret coronavirus. Some specific activity has been detected against bovine coronavirus (BCV). Clone FIPV3-70 exhibits negative reactivity against Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcivirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine Parvovirus and Canine Parainfluenza virus.
Formulation	PBS, pH 7.2 containing 0.05% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein A.
Conjugation	Unconjugated
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data