Human Aromatic L amino acid decarboxylase (DDC) ELISA Kit

Principle of the Assay: AADCS ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AADCS antibody and an AADCS-HRP conjugate. The assay sample and buffer are incubated together with AADCS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AADCS concentration since AADCS from samples and AADCS-HRP conjugate compete for the anti-AADCS antibody binding site. Since the number of sites is limited, as more sites are occupied by AADCS from the sample, fewer sites are left to bind AADCS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AADCS concentration in each sample is interpolated from this standard curve.

Intended Uses: This AADCS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AADCS. This ELISA kit for research use only, not for therapeutic or test applications!

Small volumes of DDC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7238872 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aromatic L amino acid decarboxylase (DDC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DDC. The ELISA analytical biochemical technique of the MBS7238872 kit is based on DDC antibody-DDC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DDC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DDC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).