Human G-CSF ELISA Kit
Referência ELH-GCSF-1
Tamanho : 1PlateKit
Marca : RayBiotech
Human G-CSF ELISA Kit
RayBio® Human GCSF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Sensitivity | 1 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1 pg/ml - 500 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P09919 |
| Gene Id | 1440 |
| Gene Symbols | CSF3|C17ORF33|GCSF |
| Protein Name / Synonyms | Granulocyte colony-stimulating factor (G-CSF) (Pluripoietin) (Filgrastim) (Lenograstim) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Functional expression cloning of molecules inducing CD34 expression in bone marrow-derived stromal myofibroblasts
Species:
Human
Sample Type:
Conditioned Media (nFibs)
Year:
2020
Journal Name:
Biochemical and Biophysical Research Communications
A phase II double-blinded study to evaluate the efficacy of EW02 in reducing chemotherapy-induced neutropenia in breast cancer
Species:
Human
Sample Type:
Serum (neutropenia cancer patients undergoing EW02 therapy)
Year:
2015
Journal Name:
Oncology Letters
PubMed ID:
26622752
Stretch-induced human myometrial cytokines enhance immune cell recruitment via endothelial activation
Species:
Human
Sample Type:
Conditioned Media (Stretch induced hTERT-HM cells)
Year:
2014
Journal Name:
Cellular and Molecular Immunology
Phenotypic features of first-generation transgenic goats for human granulocyte-colony stimulation factor production in milk
Species:
Human
Sample Type:
Serum (Goat produced GCSF)
Year:
2014
Journal Name:
Biotechnology Letters
Enhanced Heterologous Expression of Biologically Active Human Granulocyte Colony Stimulating Factor in Transgenic Tobacco BY-2 Cells by Localization to Endoplasmic Reticulum
Species:
Human
Sample Type:
Conditioned Media (Transfected BY-2 Tobacco cells with hGCSF.)
Year:
2014
Journal Name:
Molecular Biotechnology
Enteral Granulocyte-Colony Stimulating Factor and Erythropoietin Early in Life Improves Feeding Tolerance in Preterm Infants: A Randomized Controlled Trial
Species:
Human
Sample Type:
Serum
Year:
2014
Journal Name:
The Journal of Pediatrics
Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing
Species:
Human
Sample Type:
Tissue Lysate (Amnion and Chorion Samples)
Year:
2014
Journal Name:
Journal of Biomedical Materials Research Part B: Applied Biomaterials
PubMed ID:
25176107
Properties of dehydrated human amnion/chorion composite grafts: Implications for wound healing and soft tissue regenereation
Species:
Human
Sample Type:
Tissue Lysate (dHACM, dehydrated Human Amnion/chorion membrane)
Year:
2014
Journal Name:
Journal of Biomedical Materials Research Part B: Applied Biomaterials
PubMed ID:
24664953
Myeloproliferative neoplasia remodels the endosteal bone marrow niche into a self-reinforcing leukemic niche.
Species:
Human
Sample Type:
Conditioned Media (Myeloid-enriched BM cells)
Year:
2013
Journal Name:
Cell Stem Cell
PubMed ID:
23850243
Biological properties of dehydrated human amnion/chorion composite graft: implications for chronic wound healing.
Species:
Human
Sample Type:
Tissue Lysate (human amnion/chorion grafts)
Year:
2013
Journal Name:
International Wound Journal
PubMed ID:
23902526
- Easy-to-Use, but not specific enoughI used the RayBio Human G-CSF ELISA Kit recently to measure the amount of G-CSF in mouse serum samples. I chose this kit mainly because the standard curve was within the range of the protein levels typically seen in our samples. The kit was easy to use - everything was labeled clearly and the manual was very easy to follow and understand. The only downside of the procedure was that it was very long (~5 hours long). The only issue is that I do not think the kit was specific enough for human G-CSF. When I read the plate with our samples, there was some detection of G-CSF from the negative control that we ran, which is not supposed to happen. We think that that may be because it is detecting some mouse G-CSF within the sample. That being said, I will still definitely recommend this kit to those who are looking for human G-CSF detection in their samples.
from Particella,
on
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