OKDB00021-1x96Wells | IL-13 ELISpot Assay Kit (Human) Clinisciences

Datasheets/Manuals	Printable datasheet for IL-13 ELISpot Assay Kit (Human) (OKDB00021)

Product Info
Predicted Species Reactivity	Human
Application	ELISPOT
ELISA Kit Duration	< 4 Hours
ELISA Kit Principle	Aviva's Human IL-13 ELISpot Kit is based on standard sandwich ELISA methodology. A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtiter plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.
ELISA Kit Component	Reagents sufficient for 96 Wells: 1. Pre-coated 96 well PVDF bottomed plates (1x96 Wells) 2. Biotinylated detection antibody 3. Streptavidin-Alkaline Phosphatase conjugate 4. Bovine Serum Albumin 5. Ready to use BCIP/NBT substrate buffer
Reconstitution and Storage	2 to 8C, Do not use past expiration date.
Sample Type	Cell Suspensions
Assay Info	Assay Methodology: Quantitative Sandwich ELISpot Immunoassay

Gene Symbol	IL-13
Gene Full Name	interleukin 13
Alias Symbols	P600, IL-13
NCBI Gene Id	3596
Protein Name	interleukin-13
Description of Target	This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
Uniprot ID	P35225
Protein Accession #	NP_002179.2
Nucleotide Accession #	NM_002188.2
Protein Size (# AA)	146
Molecular Weight	16 kDa

IL-13 ELISpot Assay Kit (Human)