MAP2 ELISA Kit (Human)
Referência OKCD02710
Tamanho : 96Wells
Marca : Aviva Systems Biology
MAP2 ELISA Kit (Human) (OKCD02710)
| Datasheets/Manuals | Printable datasheet for MAP2 ELISA Kit (Human) (OKCD02710) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein 2 (MAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein 2 (MAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein 2 (MAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Microtubule Associated Protein 2 (MAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microtubule Associated Protein 2 (MAP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microtubule Associated Protein 2 (MAP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 6.4 pg/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Microtubule Associated Protein 2 (MAP2). No significant cross-reactivity or interference between Microtubule Associated Protein 2 (MAP2) and analogues was observed. | ||||||||||||||||||||||
| Publications | Expression of the microtubule-associated protein 2 (MAP2) as a potential independent prognostic marker in prostate cancer | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
| Protocol Information |
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| Gene Symbol | MAP2 |
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| Gene Full Name | microtubule associated protein 2 |
| Alias Symbols | MAP-2;MAP2A;MAP2B;MAP2C;microtubule-associated protein 2. |
| NCBI Gene Id | 4133 |
| Protein Name | Microtubule-associated protein 2 |
| Description of Target | The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. |
| Uniprot ID | P11137 |
| Protein Accession # | NP_001034627.1 |
| Nucleotide Accession # | NM_001039538.1 |
| Protein Size (# AA) | 1827 |
