Micro Dehydroascorbic Acid (DHA) Assay Kit

Principle of the Assay: Fluo-4 Calcium Assay Kit is a kit for detecting intracellular calcium concentration based on Fluo-4 AM calcium fluorescent probe. This kit can be used for fluorescence imaging detection using a fluorescence microscopy, or quantitative fluorescence detection using florescence microplate reader or flow cytometry. Using fluorescence microscopy or fluorescence microplate reader can also detect the dynamic changes of the intracellular calcium concentration. Fluo-4 is a calcium fluorescence probe that replaces Cl in the Fluo-3 structure with F. Due to the replacement of Cl with F with stronger electronic attraction, its maximum excitation wavelength will deviate by about 10 nm towards the shorter wavelength direction. This wavelength is closer to the wavelength of an argon laser, so when excited by an argon laser, the fluorescence intensity of Fluo-4 is stronger than that of Fluo-3. Fluo-4 AM penetrates the cell membrane and enters the cell, where it is cleaved by esterases within the cell to form Fluo-4, which is then trapped within the cell. When Fluo-4 exists as a free ligand, it is almost non fluorescent, but when combined with intracellular calcium, it can produce strong fluorescence. Laser confocal microscopy can be used to detect changes in intracellular calcium concentration.

Small volumes of DHA assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9719374 is an assay kit targeting DHA. Also known as Micro Dehydroascorbic Acid (DHA) Assay Kit.