Product Info Summary
| Catalog: | MEK1005 |
|---|---|
| Species: | Human |
| Sample Type: | Serum, EDTA/Heparin Plasma |
| Volume Required per Well: | Min 25 µl |
| Assay Length: | 2.25 hours |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Manufacturer Reference Number: | 110933HU |
| Analytes: | IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-23, TNFα, TNFβ |
Product info
Product Overview
The Multiplex Human Cytokine – Screen (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Multiple analyte measurements are achieved by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well of a 96-well plate. Our high-quality reagents help ensure the accuracy and precision of your results.
Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours. The Multiplex Human Cytokine – Screen (16-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample.
Assay Ranges
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| IFNγ | Sandwich | 8,000 - 10.97 (pg/mL) | 5,600 (pg/mL) | 14.3 (pg/mL) | 5.49 (pg/mL) | 8% | 4% | 97% |
| IL-1α | Sandwich | 4,000 - 5.49 (pg/mL) | 2,800 (pg/mL) | 7.1 (pg/mL) | 2.74 (pg/mL) | 6% | 7% | 86% |
| IL-1β | Sandwich | 12,226 - 16.77 (pg/mL) | 8,558 (pg/mL) | 21.8 (pg/mL) | 8.39 (pg/mL) | 8% | 5% | 94% |
| IL-2 | Sandwich | 5,213 – 7.15 (pg/mL) | 3,649 (pg/mL) | 9.3 (pg/mL) | 3.58 (pg/mL) | 6% | 4% | 99% |
| IL-4 | Sandwich | 1,676 – 2.30 (pg/mL) | 1,173 (pg/mL) | 3.0 (pg/mL) | 1.15 (pg/mL) | 6% | 6% | 94% |
| IL-5 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.3 (pg/mL) | 2.06 (pg/mL) | 10% | 7% | 95% |
| IL-6 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.3 (pg/mL) | 2.06 (pg/mL) | 7% | 4% | 104% |
| IL-8 | Sandwich | 2,000 – 2.74 (pg/mL) | 1,400 (pg/mL) | 3.6 (pg/mL) | 1.37 (pg/mL) | 12% | 7% | 100% |
| IL-10 | Sandwich | 8,039 – 11.03 (pg/mL) | 5,627 (pg/mL) | 14.3 (pg/mL) | 5.51 (pg/mL) | 7% | 5% | 103% |
| IL-12p70 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.3 (pg/mL) | 2.06 (pg/mL) | 7% | 7% | 103% |
| IL-13 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.3 (pg/mL) | 2.06 (pg/mL) | 6% | 7% | 98% |
| IL-15 | Sandwich | 10,733 – 14.72 (pg/mL) | 7,513 (pg/mL) | 19.1 (pg/mL) | 7.36 (pg/mL) | 10% | 7% | 96% |
| IL-17A | Sandwich | 16,772 – 23.01 (pg/mL) | 11,740 (pg/mL) | 29.9 (pg/mL) | 11.50 (pg/mL) | 7% | 7% | 95% |
| IL-23 | Sandwich | 41700 – 57.20 (pg/mL) | 21,000 (pg/mL) | 120 (pg/mL) | 28.60 (pg/mL) | 10% | 5% | 105% |
| TNFα | Sandwich | 6,500 – 8.92 (pg/mL) | 4,522 (pg/mL) | 11.5 (pg/mL) | 4.46 (pg/mL) | 6% | 5% | 98% |
| TNFβ | Sandwich | 4,000 – 5.49 (pg/mL) | 2,800 (pg/mL) | 7.1 (pg/mL) | 2.74 (pg/mL) | 8% | 5% | 96% |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips