PKC alpha antibody detects PKC alpha protein in the rod bipolar cells by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody (GTX130453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PKC alpha antibody detects PKC alpha protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Red: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody (GTX130453) diluted at 1:2000.

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody (GTX130453) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (GI: gastrointestinal)

PKC alpha antibody detects PKC alpha protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.