PPAR alpha antibody
Non-transfected (–) and transfected (+) unboiled HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ChIP experiment and primer designs are based on J Biol Chem. 2013 Jan 18;288(3):1726-38.
Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.








