Prolactin Rabbit Recombinant
Prolactin Rabbit Recombinant
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Product Description
Specifications
| Size | 50 µg, 1000 µg |
| Species | Rabbit |
| Accession Number | Q28632 |
| Gene Id | 100009394 |
| Protein Name / Synonyms | Mammotropin, Luteotropic hormone, Luteotropin, PRL. |
| Expressed Region | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Pro-Ile-Cys. |
| Expression System | E. coli |
| Purity | Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. |
| Activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be <, 0.065ng/ml. |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Prolactin Rabbit Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 23007 Dalton. The Porlactin is purified by proprietary chromatographic techniques.
Specifications
Protein Content
Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.87 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Prolactinl as a Reference Standard.
Stability & Storage
Storage/Stability
Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.