Recombinant Human TNF alpha Protein
Referência 230-00243-100
Tamanho : 100ug
Marca : RayBiotech
Recombinant Human TNF alpha Protein
Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
|---|---|
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P01375 |
| Gene Id | 7124 |
| Gene Symbols | TNFA |
| Protein Name / Synonyms | TNFa, TNF-alpha, Tumor necrosis factor |
| Expressed Region | Val77-Leu233 |
| Expression System | E. coli |
| Tag | C-terminal 6x histidine tag |
| Purity | > 95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 17 kDa |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial) |
| Preservative | None |
| Endotoxin Level | Not determined |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, Antibody Drug Development, Drug Antibody (DA) Detection, Protein Therapeutics, Receptor-Ligand Binding |
| Shipping Type | Blue ice |
| Storage | -20°C |
Data Images
Figure 1
Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells or HeLa cells treated with TNF-α (RayBio Cat# 230-00243). After stimulation with TNF-α, activated NF-κB p65 is translocated into the nucleus where it binds with its corresponding DNA. A. Western blotting result of NF-κB p65 from cytoplasm and nuclear fractions; B. Transcription factor activity assay of NF-κB p65 from nuclear fractions, measured by TF-ELISA kit (RayBio Cat# TFEH-p65).
Figure 2
Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells or HeLa cells treated with TNF-α (RayBio Cat# 230-00243) with the specific competitor or non-specific competitor. The result shows specific binding of NF-κB p65 to the NF-κB DNA binding site.
Figure 3
Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells treated with TNF-α (RayBio Cat# 230-00243) and purified NF-κB p50. No cross-reactivity has been observed between NF-κB p65 and p50.
