Recombinant Rat VEGF 164 Protein
Referência orb2994452-500ug
Tamanho : 500ug
Marca : Biorbyt
Description
Research Area
Cardiovascular Research; Cell Biology
Images & Validation
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Key Properties
−| Expression System | P.Pichia |
|---|---|
| Biological Origin | Rat |
| Biological Activity | ELISA: Immobilized Rat VEGF 164(Cat#orb2994452) at 2μg/ml (100 μl/well) can bind Mouse VEGFR2-Fc(Cat#orb2993886).The ED50 of Mouse VEGFR2-Fc(Cat#orb2993886) is 22.17 ng/ml (Regularly tested).. BLI: Loaded Mouse VEGFR2-Fc(Cat#orb2993886) on Protein A Biosensor, can bind Rat VEGF 164(Cat#orb2994452) with an affinity constant of 0.26 nM as determined in BLI assay. Loaded Human VEGF Antibody (5E7)-Fc(Cat#DA082) on AHC Biosensor, can bind Rat VEGF 164(Cat#orb2994452) with an affinity constant of 1.09 nM as determined in BLI assay. |
| Tag | Tag free |
| Expression Region | Ala27-Arg190(Ala36Thr) |
| Endotoxins | < 0.1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 19.2 KDa. Observed: 18-23 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Vascular endothelial growth factor A; Vascular permeability factor; VEGF; VEGF-A; VPF
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide