CUT&Tag (Cleavage Under Targets and Tagmentation) is a highly sensitive, low-input technique for profiling protein–DNA interactions and chromatin modifications with exceptional resolution and minimal background noise. The method relies on antibody-directed recruitment of a protein A/G–Tn5 transposase fusion protein, enabling in situ tagmentation and the direct generation of sequencing-ready DNA fragments from intact nuclei or permeabilized cells without the need for chromatin shearing or immunoprecipitation.
Key Advantages
- High Sensitivity and Low Input: Suitable for experiments using only hundreds to thousands of cells, including precious clinical and limited biological samples.
- High Signal-to-Noise Ratio: Delivers reduced nonspecific background and highly accurate peak identification for robust epigenomic analysis.
- Broad Sample Compatibility: Applicable to primary cells, sorted cell populations, frozen specimens, lightly fixed samples, and single-cell workflows.
- Streamlined Workflow: Minimizes handling steps, reducing sample loss, experimental variability, and processing time.
Applications
- Epigenomic mapping of histone modifications and transcription factor binding sites.
- Comparative chromatin profiling across cell types, treatments, developmental stages, or disease conditions.
- Low-input epigenetic studies and clinical sample analysis.
- Single-cell and indexed CUT&Tag applications for cell-type-specific chromatin landscape characterization.
