Tamanho : 50mg
Synonyms: WR-256283
| Description | Artesunate (WR-256283) is a semisynthetic derivative of artemisinin and an inhibitor of STAT-3/EXP1. Water-soluble yet relatively unstable in neutral or acidic aqueous solutions, Artesunate is primarily employed for the treatment of malaria. |
| In vitro | Methods: SKM-1 cells were treated with 0–50 μg/mL Artesunate for 48 h. Methylation-specific PCR was used to detect CDH1 methylation status, while Western blot and laser confocal microscopy were employed to detect E-cadherin expression and localization. Results: Artesunate treatment induced CDH1 demethylation, restored E-cadherin expression, and promoted its aggregation to the cell membrane.[1] Methods: Human glioma SHG44 cells were treated with 0–50 mg/L Artesunate for 24–48 h. Cell proliferation was detected by MTT assay, apoptosis by flow cytometry, migration and invasion by Transwell assay, biomechanical properties by atomic force microscopy, and claudin-1 expression by RT-PCR and Western blot. Results: Treatment with 30 mg/L Artesunate for 48 h significantly inhibited cell proliferation, migration, and invasion, induced apoptosis rate to 23.7%, while increasing cell adhesion force and membrane roughness, decreasing elasticity, and upregulating claudin-1 expression.[2] |
| In vivo | Methods: HeLa cell xenograft nude mice were randomly divided into groups. The Artesunate group received daily intraperitoneal injection of 100 mg/kg (diluted in sterile PBS) for 7 consecutive days, while the irradiation group received 10 Gy irradiation on day 7. Results: Combined treatment reduced tumor volume by 72.34%, significantly higher than 41.22% with irradiation alone, confirming the in vivo radiosensitizing effect of ART.[3] |
| Synonyms | WR-256283 |
| Cell Research | Growth inhibition assay: The SKM-1 cells (1×105/mL) are firstly seeded in 96-well plates. Artesunate is diluted in 0.1% dimethyl sulfoxide (DMSO) producing 0, 12.5, 25, 50µg/mL concentrations and added to the SKM-1 cells with 100 µl per well. A negative control is treated with 0.1% DMSO. At 0, 24, 48, and 72 hours, same amount of MTT solution is added into each well and cultured for extra 4 hours. MTT treated cells are fixed with 150 µL DMSO for 30 min at room temperature and then determined with Evolution 201 and 220 UV-Vis spectrophotometer system at 540 nm. (Only for Reference) |
| Molecular Weight | 384.42 |
| Formula | C19H28O8 |
| Cas No. | 88495-63-0 |
| Smiles | C[C@@H]1[C@]2([C@]34[C@](O[C@H]1OC(CCC(O)=O)=O)(O[C@](C)(OO3)CC[C@]4([C@H](C)CC2)00)00)00 |
| Relative Density. | 1.31 g/cm3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
| Solubility Information | Ethanol: 9 mg/mL (23.41 mM), Sonication is recommended. H2O: < 1 mg/mL (insoluble or slightly soluble) DMSO: 250 mg/mL (650.33 mM), Sonication is recommended.  | ||||||||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (13.01 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
Ethanol/DMSO
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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