Avidin Antibody Peroxidase Conjugated
Referência orb344923-25ul
Tamanho : 25ul
Marca : Biorbyt
Description
Images & Validation
−
| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:2,000 - 1:10,000, IHC: 1:250 - 1:1,000, WB: 1:1,000 - 1:2,500 |
| Reactivity | Bacteria |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Avidin (Hen Egg White) |
| Purity | Anti-Avidin Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White ]. Cross reactivity against Avidin from other sources is unknown. |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Avidin Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Avidin antibody, Avidin Peroxidase, Anti-Avidin HRP Antibody
−
Mouse Human IgA1+IgA2 , conjugated with Horseradish peroxidase Antibody [orb22225]
Human
Mouse
Monoclonal
HRP
0.2 mgAvidin Antibody Peroxidase Conjugated [orb344921]
DOT, ELISA, IHC, WB
Bacteria
Rabbit
Polyclonal
HRP
20 mgAvidin Antibody Peroxidase Conjugated [orb344922]
DOT, ELISA, IHC, WB
Bacteria
Rabbit
Polyclonal
HRP
100 μg
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot