Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.

NGF Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 13.2 kDa and the accession number is P01138.

Human

CHO Cells

β-NGF,NGFB,nerve growth factor (β polypeptide),nerve growth factor (beta polypeptide),HSAN5,Beta-NGF

A DNA sequence encoding the mature form of human β-NGF (NP_002497.2) (Ser 122-Arg 239) was expressed. Predicted N terminal: Ser 122

> 95 % as determined by SDS-PAGE

Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.