Snippet: ic> for 2 min at 4°C. Isolated RNA samples were stored at −80°C until further use. cDNA was synthesized from total RNA using the cDNA Synthesis Kit according to the manufacturer's instructions (Origene Technology, HP100042, USA). Synthesized cDNA samples were stored at

Snippet: …The reverse transcription of total miR-212 was performed using cDNA Synthesis Kit for miRNA (Origene, Kwun Tong, Hong Kong, #HP100042) according to the manufacturer̵s protocols. … The reverse transcription of total miR-212 was performed using cDNA Synthesis Kit for miRNA (Origene, Kwun Tong, Hong Kong, #HP100042) according to the manufacturer’s protocol. …

Snippet: erse Transcription Supermix (cat. No. 1708840, BioRad) for mRNA and qSTAR miRNA qPCR Detection System (cat. No. HP100042, OriGene Technologies) for miRNA, following the manufacturer’s instructions. The cDNA samples and SYBR Green PCR Master Mix (Applied Bi

Snippet: nstrated that microRNAs (miRNAs) are aberrantly expressed in various types of cancer, such as acute myeloid leukemia (AML), oral, breast, lung, and colorectal cancer, and that they are critical in tumorigenesis and tumor metastasis [23–25]. miRNAs are a class of small non-coding RNA with a length ranging from 18 to 24 nucleotides, and they usually function as negative regulators of gene expressi;and from miRNA using a qSTAR miRNA qPCR detection system (HP100042, OriGene, Rockville, MD, USA)