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> Dextran [9004-54-0]

Dextran [9004-54-0]

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Referência HY-112624-10g

Tamanho : 10g

Marca : MedChemExpress


Description

Dextran (Dextran 40) has an inhibitory effect on thrombocyte aggregation and coagulation factors and is used as a plasma volume expander.

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Dextran can be used in animal modeling to create paw edema models. Injection of dextran at doses of either 7.9 mg/kg or 300 mg/kg results in edema of equal intensity in the limbs[1][2].

Induction of Paw Edema[1][2].
Background
Dextran promotes the release of histamine and serotonin from mast cells during edema formation. These substances alter the tone and vascular permeability, contributing to fluid extravasation[1][2].
Specific Modeling Methods
Mice: Swiss albino• male • weighing 25-30 g[1].
Administration: 1% dextran (20 μL/paw)• intraplantar injection in the right hind paw• a single dose for 1, 2, 3 and 4 h[1].
Rat: Albino of of the Sprague Dawleq strain• weighing 110-130 g• seven-week-old[2].
Administration: 0.5 mL/100 g • tail vein injection• a single dose[2].
Note
(1)Dextran (6% v/w in saline) was freshly diluted each day so as to contain 7.9 mg or 600 mg per 5 ml volume of physiological saline[2].
Modeling Indicators
Morphological change: Developed marked inflammatory reaction and significantly increased the volume of paw edema[1].
Histological analysis: Mast-cell disruption (histamine and serotonili release), vasodilatation, hypothermia, hypotension, helnoconcentration and edema occurred with the high doses of Dextran. Since minute doses of dextran induced only vasodilatation and edema formation[2].
Correlated Product(s): Carrageenan (HY-125474); Histamine (HY-B1204); Arachidonic acid (HY-109590)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Essai clinique
Masse moléculaire

40000.00

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Dextran]
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvant et solubilité
In Vitro: 

H2O : 100 mg/mL (2.50 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.0250 mL 0.1250 mL 0.2500 mL
5 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation
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