ENPP2 ELISA Kit (Mouse)
Referência OKEH03320
Tamanho : 96Wells
Marca : Aviva Systems Biology
ENPP2 ELISA Kit (Mouse) (OKEH03320)
| Datasheets/Manuals | Printable datasheet for ENPP2 ELISA Kit (Mouse) (OKEH03320) |
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| COA Datasheet | Printable COA datasheet for OKEH03320 |
| Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology ENPP2 ELISA Kit (Mouse) (OKEH03320) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Enpp2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Enpp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Enpp2 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.78-50ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <= 8.3% (n = 20) Mean Inter-assay CV%: <= 9.8% (n = 20) | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.39 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Enpp2 |
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| Gene Full Name | ectonucleotide pyrophosphatase/phosphodiesterase 2 |
| Alias Symbols | AT, ATX, Auto, autotaxin, E-NPP 2, ectonucleotide pyrophosphatase/phosphodiesterase family member 2, extracellular lysophospholipase D, lysoPLD, N, Npps2, Pd, PD-, PD-Ialpha, Pdnp2, phosphodiesterase I/nucleotide pyrophosphatase 2. |
| NCBI Gene Id | 18606 |
| Protein Name | Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 |
| Description of Target | Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:17208043, PubMed:28414242, PubMed:27780639). Major substrate is lysophosphatidylcholine (PubMed:17208043, PubMed:27780639). Also can act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:18175805). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition (By similarity). Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor. |
| Uniprot ID | Q9R1E6 |
| Protein Accession # | NP_001129549.1 |
| Nucleotide Accession # | NM_001136077.3 |
| Protein Size (# AA) | 862 |
| Molecular Weight | 99 kDa |