Histone H4, acetylated
Referência MBS616728-0,2mL
Tamanho : 0.2mL
Marca : MyBiosource
WB: Acid extract of untreated (lane 1) or butyrate-treated (lane 2) HeLa cells probed with MBS616728 Histone H4 at 1:2000 dilution.
Sonicated chromatin prepared from untreated HeLa cells (1x10e6 cell equivalents) was subjected to chromatin immunoprecipitation using 4uL of either a normal rabbit antiserum or Acetyl Histone H4 serum and the Magna ChIP A Kit. Successful immunoprecipitation of acetyl histone H4 associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human GAPDH promoter.
Histone H4, Acetylated (HIST4H4) Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS616728MBS616728 is an antibody targeting HIST4H4. Also known as Histone H4, acetylated. Reactive in Human, Th. Host: Rabbit. More details
Product Overview
Online Datasheet in PDFProduct Name
Histone H4, acetylated (HIST4H4), Polyclonal Antibody
Full Product Name
Histone H4, acetylated
Product Gene Name
anti-HIST4H4 antibody
Product Synonym Names
Anti -Histone H4, acetylated
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Th
Form/Format
Suppied as frozen neat serum, 0.05% sodium azide.
Purity/Purification
Serum
Serum
Serum
Specificity
Recognizes acetylated histone H4 of approximately 10kD. Cross-reacts with acetylated
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
11,367 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (14923654..14924065, complement). Location: 12p12.3
OMIM
142750
Immunogen
KLH-conjugated peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS-C] corresponding to amino acids 2-19 of Tetrahymena histone H4.
Product Categories/Family for anti-HIST4H4 antibody
Antibodies; Abs to Histones
Applications Tested/Suitable for anti-HIST4H4 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-HIST4H4 antibody
Western Blotting: 1:2000 dilution detects acetylated histone H4 in Tetrahymena macronuclei and acid extracted proteins from HeLa cells treated with 5mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4., Chromatin Immunoprecipitation: 5-10ul immunoprecipitates transcriptionally active, chromatin containing acetylated histone H4 from 2X 106 serum stimulated HeLa cells., Immunocytochemistry: Not recommended.
Immunoprecipitation (IP) of anti-HIST4H4 antibody
Sonicated chromatin prepared from untreated HeLa cells (1x10e6 cell equivalents) was subjected to chromatin immunoprecipitation using 4uL of either a normal rabbit antiserum or Acetyl Histone H4 serum and the Magna ChIP A Kit. Successful immunoprecipitation of acetyl histone H4 associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human GAPDH promoter.
Other Notes
Small volumes of anti-HIST4H4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for HIST4H4. It may not necessarily be applicable to this product.
NCBI Official Full Name
histone H4
NCBI Official Synonym Full Names
histone cluster 4, H4
NCBI Official Symbol
HIST4H4
NCBI Official Synonym Symbols
H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
UniProt Protein Name
Histone H4
UniProt Gene Name
HIST1H4A
UniProt Synonym Gene Names
H4/A; H4FA
UniProt Entry Name
H4_HUMAN
NCBI Protein Information
histone H4; histone 4, H4; OTTHUMP00000239323
NCBI Summary for HIST4H4
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq]
Show more UniProt Comments for HIST4H4
H4: histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The gene encoding H4 is intronless.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 6p22.2
Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus
Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity
Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly at centromere; DNA replication-independent nucleosome assembly; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; mitotic cell cycle; DNA replication-dependent nucleosome assembly; telomere maintenance; DNA methylation on cytosine; regulation of gene expression, epigenetic; negative regulation of megakaryocyte differentiation
Show more Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 6p22.2
Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus
Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity
Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly at centromere; DNA replication-independent nucleosome assembly; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; mitotic cell cycle; DNA replication-dependent nucleosome assembly; telomere maintenance; DNA methylation on cytosine; regulation of gene expression, epigenetic; negative regulation of megakaryocyte differentiation
Research Articles on HIST4H4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with anti-HIST4H4 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Amyloids Pathway antibodies | Amyloids Pathway Diagram |
| Chromosome Maintenance Pathway antibodies | Chromosome Maintenance Pathway Diagram |
| Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway antibodies | Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway Diagram |
| Meiotic Recombination Pathway antibodies | Meiotic Recombination Pathway Diagram |
| Nucleosome Assembly Pathway antibodies | Nucleosome Assembly Pathway Diagram |
| Packaging Of Telomere Ends Pathway antibodies | Packaging Of Telomere Ends Pathway Diagram |
| RNA Polymerase I Chain Elongation Pathway antibodies | RNA Polymerase I Chain Elongation Pathway Diagram |
| RNA Polymerase I Promoter Clearance Pathway antibodies | RNA Polymerase I Promoter Clearance Pathway Diagram |
| RNA Polymerase I Promoter Opening Pathway antibodies | RNA Polymerase I Promoter Opening Pathway Diagram |
| RNA Polymerase I Transcription Pathway antibodies | RNA Polymerase I Transcription Pathway Diagram |
Diseases associated with anti-HIST4H4 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Stomach Diseases Antibodies | >1 publications with HIST4H4 and Stomach Diseases |
| Stomach Neoplasms Antibodies | >1 publications with HIST4H4 and Stomach Neoplasms |
| Death Antibodies | >1 publications with HIST4H4 and Death |
| Neoplasms Antibodies | >1 publications with HIST4H4 and Neoplasms |