Human CD14 Antibody : LE/AF
Referência OASB01059
Tamanho : 0.5mg
Marca : Aviva Systems Biology
Human CD14 Antibody : LE/AF (OASB01059)
| Datasheets/Manuals | Printable datasheet for Human CD14 Antibody : LE/AF (OASB01059) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | 61D3 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, ICC, WB, CT, BL |
| Additional Information | Description: CD14 is a 55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells, and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | Human peripheral monocytes |
| Concentration | 0.5 mg/mL |
| Specificity | CD14 |
| Characterization | To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate PE/CY5.5 and PE/CY7 conjugates APC/CY5.5 and APC/CY7 conjugates AF488 and AF647 conjugates <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry, Immunohistochemistry (frozen sections), Immunoprecipitation |
| Other Applications Data | Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), Alexa Fluor 488 (AF488), and Alexa Fluor 647 (AF647) conjugates are suppliedas 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- PE conjugate and allophycocyanin conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3and a stabilizing agent; store at 2- 8 C - The low endotoxin/azide- free antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS;aliquot and freeze upon receipt - The Spectral Red (SPRD), R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5), R- phycoerythrin- Cyanine 7(R- PE- CY 7), Allophycocyanin- Cyanine 5.5 (APC- CY 5.5), and Allophycocyanin- Cyanine 7 (APCCY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at2- 8 C. Do not freeze! - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
| Reference | 1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: WhiteCell Differentiation Antigens, Oxford University Press, Oxford 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD14 Section, Academic Press, New York, p. 169 3. Hogg, N., S. MacDonald, M. Slusarenko, and P.C. Beverley. 1984. Immunol. 53:753 4. Stocks, S.C., M. Albrechtsen, and M.A. Kerr. 1990. Biochem. J. 268:275. |
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| Gene Symbol | CD14 |
| Gene Full Name | Monocyte differentiation antigen CD14 |
| NCBI Gene Id | 929; 102129342; 607076 |
| Protein Name | monocyte differentiation antigen CD14 |
| Description of Target | The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. |
| Uniprot ID | P08571, B3Y6B7, A9QU57 |
| Protein Accession # | NP_000582.1 |
| Nucleotide Accession # | NM_000591.3 |
