RayBio® C-Series Human Cytokine Antibody Array 9 Kit. Detects 51 Human Cytokines. Suitable for all liquid sample types.
Human Cytokine Array C9
Human Cytokine Array C9
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 51 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Adipsin (Complement Factor D)
| BCAM
| CD30 (TNFRSF8)
| CD40 (TNFRSF5)
| Fc gamma RIIB/C (CD32B/C)
|
Ferritin
| FLRG
| Follistatin
| Furin
| Galectin-7
|
GDF-15
| Growth Hormone
| IL-10 R alpha
| IL-22
| IL-28A (IFN-lambda 2)
|
IL-29 (IFN-lambda 1)
| IL-31
| Insulin
| Luteinizing hormone
| LIMPII
|
LYVE-1
| Marapsin
| MICA
| MICB
| MMP-2
|
MMP-7
| MMP-8
| MMP-10
| NCAM-1 (CD56)
| Nidogen-1
|
NrCAM
| NRG1-beta 1 (HRG1-beta 1)
| Osteopontin (SPP1)
| PAI-1
| Platelet factor 4 (CXCL4)
|
PSA-total
| RAGE
| RANK (TNFRSF11A)
| Resistin
| SAA (Serum Amyloid A)
|
Siglec-9
| TACE
| TIM-1 (KIM-1)
| TRAIL R2 (TNFRSF10B/DR5)
| Trappin-2
|
TREM-1
| TSH
| TSLP
| VCAM-1 (CD106)
| VEGF-C
|
XEDAR
| ||||
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C9 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
The efficacy of pegylated interferon alpha-2a and entecavir in HBeAg-positive children and adolescents with chronic hepatitis B
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Bmc Pediatrics
Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal Adenocarcinoma
- Species:
- Human
- Sample Type:
- Conditioned Media (HPS, Hypoxic PS1 supernates)
- Year:
- 2020
- Journal Name:
- Biomedicines
p16INK4A Inhibits the Pro-Metastatic Potentials of Osteosarcoma Cells Through Targeting the ERK Pathway and TGF-b1
- Species:
- Human
- Sample Type:
- Conditioned Media (Primary normal human skin fibroblast cells, Osteosarcoma cell line U2OS, and EH1 derivation of U20S expressing p16)
- Year:
- 2015
- Journal Name:
- Molecular Carcinogenesis
- PubMed ID:
- 25728247
GDF-15 secreted from human umbilical cord blood mesenchymal stem cells delivered through the cerebrospinal fluid promotes hippocampal neurogenesis and synaptic activity in an Alzheimer's disease model
- Species:
- Human
- Sample Type:
- Conditioned Media (HUVEC and MSCs in alzheimer's)
- Year:
- 2015
- Journal Name:
- Stem Cells and Development
- PubMed ID:
- 26154268
Cancer-associated fibroblasts regulate the plasticity of lung cancer stemness via paracrine signalling
- Species:
- Human
- Sample Type:
- Conditioned Media (Cancer Associated Fibroblasts and Lung cancer stem cells)
- Year:
- 2014
- Journal Name:
- Nature Communications
Identification and validation of novel contraction-regulated myokines released from primary human skeletal muscle cells
- Species:
- Human
- Sample Type:
- Conditioned Media (Primary human myotubes)
- Year:
- 2013
- Journal Name:
- Plos One
- PubMed ID:
- 23637948
Circulating angiogenic cells can be derived from cryopreserved peripheral blood mononuclear cells
- Species:
- Human
- Sample Type:
- Conditioned Media (PBMCs cultured post cryopreservation)
- Year:
- 2012
- Journal Name:
- Plos One
- PubMed ID:
- 23133548
Tumor Self-Seeding by Circulating Cancer Cells
- Species:
- Human
- Sample Type:
- Conditioned Media (Cell-conditioned media)
- Year:
- 2009
- Journal Name:
- Cell