Human RTK Phosphorylation Array C1
Referência AAH-PRTK-1-2
Tamanho : 2SampleKit
Marca : RayBiotech
Human RTK Phosphorylation Array C1
RayBio® C-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Human RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 71 |
| Recommended Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Post-Translational Modifications, Phosphorylation, PI3K-AKT Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| ABL1
| ACK
| ALK-1
| Axl
| Blk
|
| BMX
| Btk
| Csk
| Dtk
| EGFR
|
| EphA1
| EphA2
| EphA3
| EphA4
| EphA5
|
| EphA6
| EphA7
| EphA8
| EphB1
| EphB2
|
| EphB3
| EphB4
| EphB6
| ErbB2
| ErbB3
|
| ErbB4
| FAK
| FER
| FGFR1
| FGFR2
|
| FGFR2 alpha isoform
| Fgr
| FRK
| Fyn
| Hck
|
| HGFR
| IGF-1 R
| Insulin R (CD220)
| Itk
| JAK1
|
| JAK2
| JAK3
| LCK
| LTK
| Lyn
|
| MATK
| M-CSF R
| MUSK
| NGFR (TNFRSF16)
| PDGFRA
|
| PDGFRB
| PYK2
| RET
| ROR1
| ROR2
|
| ROS
| RYK
| SCF R (CD117/c-kit)
| SRMS
| SYK
|
| Tec
| Tie-1
| Tie-2
| TNK1
| TRKB
|
| TXK
| Tyk2
| TYRO10 (DDR2/TKT)
| VEGFR2
| VEGFR3
|
| ZAP70
| ||||
Typical Data
Kit Components
- Human RTK Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Anti-Phospho Tyrosine Detection Antibody
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer (1 x 5 ml)
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail II
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Additional Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Assay Procedure Summary
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
