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> Human zonulin ELISA kit

Human zonulin ELISA kit

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Referência CSB-EQ027649HU-96T

Tamanho : 96T

Marca : Cusabio

SDS

CSB-EQ027649HU
Size 96T,5×96T,10×96T

Product Details

Target Name
zonulin
Abbreviation
zonulin
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human zonulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-97  
1:2 Average % 94  
Range % 89-98  
1:4 Average % 96  
Range % 93-105  
1:8 Average % 94  
Range % 86-98  
Recovery
The recovery of human zonulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-101  
EDTA plasma (n=4) 97 90-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.148 2.066 2.107 2.008  
20 1.455 1.353 1.404 1.305  
10 0.867 0.895 0.881 0.782  
5 0.557 0.571 0.564 0.465  
2.5 0.393 0.375 0.384 0.285  
1.25 0.264 0.276 0.270 0.171  
0.625 0.195 0.199 0.197 0.098  
0 0.098 0.099 0.099 　  
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human zonulin antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • Biotin-labeled zonulin antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
  • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
  • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
  • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
  • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
  • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microtiter plate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

The human zonulin ELISA kit is a valuable tool for detecting zonulin levels in human samples, such as serum, plasma, and tissue homogenates. With a detection range of 0.625 ng/mL to 40 ng/mL and a sensitivity of 0.156 ng/mL, it offers a precise measurement of zonulin concentrations. The assay principle is quantitative, utilizing a sandwich measurement technique that ensures accurate and reliable results. Researchers can perform the assay within a reasonable timeframe, as the assay time ranges from 1 to 5 hours. The sample volume required for analysis falls between 50-100 µl, making it suita...

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