IgG (lambda chain specific) (Texas Red)

Referência I1904-67E-1mg

Tamanho : 1mg

Marca : US Biological



I1904-67E IgG (lambda chain specific) (Texas Red)

Clone Type
Polyclonal
Host
rabbit
Source
mouse
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IF WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in FLISA, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt. Reconstitute with sterile ddH2O. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Texas Red conjugates are sensitve to light.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~1mg/ml|Form: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Texas Red™ Sulfonyl Chloride (Molecular Weight 625 daltons)|Absorption Wavelength: 596 nm Emission Wavelength: 620 nm. Fluorochrome/Protein Ratio: 2.5 moles Texas Red per mole of Rabbit IgG. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol for long term storage.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Mouse (Lambda light chain)|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse (Lambda light chain)
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Texas Red™ Sulfonyl Chloride (Molecular Weight 625 daltons)|Absorption Wavelength: 596 nm Emission Wavelength: 620 nm. Fluorochrome/Protein Ratio: 2.5 moles Texas Red per mole of Rabbit IgG. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol for long term storage.
Purity
Purified by immunoaffinity chromatography.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
References
J.A. Titus, P.P. Haugland, S.D. Sharrow, D.M. Segal J. Immunol. Methods 50; 193, 1982.